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csb e07007h  (Cusabio)


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    Cusabio csb e07007h
    Csb E07007h, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/endothelin+1+et+1/Human+Endothelin+1%2CET-1+ELISA+Kit/pm41500376-167-44-46
    Average 93 stars, based on 10 article reviews
    csb e07007h - by Bioz Stars, 2026-09
    93/100 stars

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    Capsules:

    Article Title: Study on the mechanism of stir-fried Fructus Tribuli in enhancing the essential hypertension treatment by an integrated "spectrum-effect relationship-network pharmacology-metabolomics" strategy.
    Article Snippet: .. Valsartan capsules (specifications, 80 mg) were purchased from Beijing Novartis Pharmaceutical Co., Ltd. Enzyme-linked immunosorbent assay (ELISA) kits of Ang II and endothelin-1 (ET-1) were purchased from Cusabio Biotech Co., Ltd. (Wuhan, China); tumor necrosis factor-alpha (TNF-α) ELISA kit from Thermo Fisher Scientific (Fair Lawn, New Jersey, USA); thromboxane B2 (TXB2) ELISA kit from Jiangsu Enzyme Industrial Co., Ltd. (Yancheng, Jiangsu, China); prostaglandin-endoperoxide synthase 1 (PTGS1) and prostaglandinendoperoxide synthase 2 (PTGS2) ELISA kits from AndyGenen Biotechnology Co., Ltd. (Beijing, China); NO kit from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). .. The transforming growth factor β1 (TGF-β1) antibody (Rabbit polyclonal to TGF-β1, 1:200) and rabbit anti-Smad3 antibody (1:200) were purchased from Bioss (Beijing, China).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Study on the mechanism of stir-fried Fructus Tribuli in enhancing the essential hypertension treatment by an integrated "spectrum-effect relationship-network pharmacology-metabolomics" strategy.
    Article Snippet: .. Valsartan capsules (specifications, 80 mg) were purchased from Beijing Novartis Pharmaceutical Co., Ltd. Enzyme-linked immunosorbent assay (ELISA) kits of Ang II and endothelin-1 (ET-1) were purchased from Cusabio Biotech Co., Ltd. (Wuhan, China); tumor necrosis factor-alpha (TNF-α) ELISA kit from Thermo Fisher Scientific (Fair Lawn, New Jersey, USA); thromboxane B2 (TXB2) ELISA kit from Jiangsu Enzyme Industrial Co., Ltd. (Yancheng, Jiangsu, China); prostaglandin-endoperoxide synthase 1 (PTGS1) and prostaglandinendoperoxide synthase 2 (PTGS2) ELISA kits from AndyGenen Biotechnology Co., Ltd. (Beijing, China); NO kit from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). .. The transforming growth factor β1 (TGF-β1) antibody (Rabbit polyclonal to TGF-β1, 1:200) and rabbit anti-Smad3 antibody (1:200) were purchased from Bioss (Beijing, China).

    Article Title: Effect of Lower- versus Higher-Intensity Isometric Handgrip Training in Adults with Hypertension: A Randomized Controlled Trial
    Article Snippet: Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) levels were measured using the enzyme-linked immune sorbent assay (ELISA) (Diaclone, Besançon, France). .. The ELISA method and special kits were used to measure Endothelin-1 (ET-1) (CUSABIO Biotechnology Company, Tokyo, Japan), malondialdehyde (MDA), and total antioxidant capacity (TAC) (ZellBio GmbH, Ulm, Germany). .. Levels of carbonyl protein (CP) were measured using a chemical colorimetric assay kit (Cayman Chemical Company, Ann Arbor, MI, USA).

    Article Title: A Three-Dimensional Microfluidic Device for Monitoring Cancer and Chemotherapy-Associated Platelet Activation
    Article Snippet: After washing, Alexa Fluor 546 goat anti-mouse antibody solution diluted in 1/200 was incubated at 37 °C in the dark for 2 h. HUVECs were also stained with 100 μL of Alexa Fluor 488-conjugated phalloidin (#P5285, Sigma-Aldrich) solution for 1 h in the dark at room temperature followed by infusing DAPI (#C1005, Beyotime) solution for 2 min. After washing, images were captured using a microscope with a CCD camera. .. The endothelin-1 (ET-1) (#CSB-E08322h, CUSABIO) and eNOS (#CSB-E07007h, CUSABIO) level from the HUVEC culture medium in each sample were measured using a commercial enzyme immunoassay technique-based kit. .. The plate was read for absorbance at 450 nm on a microplate spectrophotometer (GENios Plus, Tecan), and samples were compared to the obtained standard curve.

    Multiple Displacement Amplification:

    Article Title: Effect of Lower- versus Higher-Intensity Isometric Handgrip Training in Adults with Hypertension: A Randomized Controlled Trial
    Article Snippet: Interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) levels were measured using the enzyme-linked immune sorbent assay (ELISA) (Diaclone, Besançon, France). .. The ELISA method and special kits were used to measure Endothelin-1 (ET-1) (CUSABIO Biotechnology Company, Tokyo, Japan), malondialdehyde (MDA), and total antioxidant capacity (TAC) (ZellBio GmbH, Ulm, Germany). .. Levels of carbonyl protein (CP) were measured using a chemical colorimetric assay kit (Cayman Chemical Company, Ann Arbor, MI, USA).

    Nitric Oxide Assay:

    Article Title: Decompression Mechanism of Radish Seed in Prehypertension Rats through Integration of Transcriptomics and Metabolomics Methods.
    Article Snippet: .. Measurement of Endothelin-1 (ET-1) and NO. Te levels of ET-1 and NO in the rat sera were measured using a specifc Rat Endothelin-1 Enzyme-Linked Immunosorbent Assay Kit (Lot: G14012112, Wuhan Cusabio Co., Ltd., China) and Nitric Oxide Assay Kit (Lot: 20210604, Nanjing Jiancheng Bioengineering Institute, China) as per the manufacturer’s instructions. ..



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    Image Search Results


    Ca² + and endothelin-1 (ET-1) contribute to Piezo1-dependent activation of hypoxia-inducible factor 1-alpha (HIF-1α). a) and b) Gene set enrichment analysis (GSEA) of epidural scar tissue from mice after laminectomy. c) Ca² + influx measured by Fluo-4 AM in the blank, type I rat tail collagen-encapsulated, and collagen-encapsulated with GsMTX4 groups. d) Western blot analysis of NIH/3T3 fibroblasts treated with type I rat tail collagen and the calcium influx blocker Nimodipine (n = 3). e) Western blot analysis of NIH/3T3 fibroblasts treated with Yoda1 and Nimodipine (n = 3). f) Quantitative analysis of relative Edn1 messenger RNA (mRNA) expression in blank and collagen-encapsulated NIH/3T3 fibroblasts. g) and h) Western blot analysis of NIH/3T3 fibroblasts treated with type I rat tail collagen and the ET-1 inhibitor Bosentan (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, independent-samples t -test (two groups) or analysis of variance (multiple groups).

    Journal: Bone & Joint Research

    Article Title: Piezo1 drives fibroblast activation in epidural fibrotic remodelling via the ET-1/HIF-1α pathway

    doi: 10.1302/2046-3758.158.BJR-2025-0662.R1

    Figure Lengend Snippet: Ca² + and endothelin-1 (ET-1) contribute to Piezo1-dependent activation of hypoxia-inducible factor 1-alpha (HIF-1α). a) and b) Gene set enrichment analysis (GSEA) of epidural scar tissue from mice after laminectomy. c) Ca² + influx measured by Fluo-4 AM in the blank, type I rat tail collagen-encapsulated, and collagen-encapsulated with GsMTX4 groups. d) Western blot analysis of NIH/3T3 fibroblasts treated with type I rat tail collagen and the calcium influx blocker Nimodipine (n = 3). e) Western blot analysis of NIH/3T3 fibroblasts treated with Yoda1 and Nimodipine (n = 3). f) Quantitative analysis of relative Edn1 messenger RNA (mRNA) expression in blank and collagen-encapsulated NIH/3T3 fibroblasts. g) and h) Western blot analysis of NIH/3T3 fibroblasts treated with type I rat tail collagen and the ET-1 inhibitor Bosentan (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, independent-samples t -test (two groups) or analysis of variance (multiple groups).

    Article Snippet: Alternatively, the following drugs were used to stimulate NIH/3T3 cells: Yoda1 (25 μM; HY-P1410), a chemical activator of Piezo1; BAY 87-2243 (1 μM; HY-15836), an inhibitor of hypoxia-inducible factor 1-alpha (HIF-1α); SB-431542 (10 μM; HY-10431), an inhibitor of TGF-β1; SIS 3 (10 μM; HY-13013), an inhibitor of Smad3; GsMTX4 (2.5 μM; HY-P1410); Nimodipine (60 μM; HY-B0265), a calcium channel blocker; and Bosentan (1 μM; HY-A0013, all MedChemExpress), an endothelin-1 (ET-1) receptor antagonist.

    Techniques: Activation Assay, Western Blot, Expressing